(, Guinebretiere B. cereus contamination carried by zucchinis calls for particular attention, as it is composed to a large extent of psychrotrophic strains. M.S. Ekelund Hence it is important to monitor the potential sources of this pathogen in … However, the fact that 80% of the ingredient strains (16 strains out of 20) were clustered in only two groups at 90% similarity level (Fig. Detection of hblA and bal Genes in Bacillus cereus Isolates From Cheese Samples Using the Polymerase Chain Reaction. 2). Bacillus (Latin "stick") is a genus of Gram-positive, rod-shaped bacteria, a member of the phylum Firmicutes, with 266 named species.The term is also used to describe the shape (rod) of certain bacteria; and the plural Bacilli is the name of the class of bacteria to which this genus belongs. There is a marked seasonal variation in the spore content of raw milk, with higher levels during the pasture period, when the teats of the cow may be … Tingey Bacillus cereus. Molin A.R. Bactéries sporulées et biofilms : un problème récurrent dans les lignes de production de lait reconstitué ou recombiné pasteurisé. and Its Toxins in Pasteurized Fluid Milk In isolates from three pasteurized milk samples two different strains were detected. Optimization of washing procedures and heat treatments applied to zucchinis would help decrease contamination. Its endospore can survive mild heat treatment applied during food processing and some strains have the ability to grow at low temperatures during storage of food products. Cells (∼10 μl) were suspended in 1 ml of sterile Milli-Q water, pelleted at 11 000×g for 15 min, and resuspended in 500 μl extraction buffer (1.7% sodium dodecyl sulfate, 200 mM Tris–HCl (pH 8), 20 mM EDTA, 200 mM NaCl). Bacillus cereus DSM 4312 is a mesophilic bacterium that was isolated from food poisoning incident. Description: Bacillus cereus (B. cereus) is a Gram-positive aerobic, motile, spore-forming, rod-shaped bacterium that is widely distributed in the environment. As an exception, four milk protein strains were grouped with soil/zucchini strains. Masson The number of DNA bands detected varied from five to 12 bands ranging between 100 and 4000 bp for M13-PCR patterns and from four to nine bands ranging between 150 and 1200 bp for DAF patterns. Lanes 1, 15, 30: DNA molecular mass marker X (Roche). The strains were grown in a water bath stabilized at 4±0.2°C, 7±0.1°C, 10±0.1°C and 30°C, in 20-ml tubes containing 5 ml of J-Broth [22] supplemented with 0.1% of agar and hermetically sealed. Smith Bacillus cereus and Bacillus licheniformis are the predominant pathogenic Bacillus species found in raw milk and at all stages of dairy processing (Kalogridou‐Vassiliadou 1992; Scheldeman et al. 16 combined profile groups and six unclustered strains were formed at 75% similarity level. Combination of M13-PCR and DAF techniques provided discriminative and informative results for tracing the spread of B. cereus contamination in a food plant. The 134 strains were typed with both methods. Among five yoghurts, there was no dominant strain: the samples carried three different strains. This study showed which of the four potential sources of contamination contributed to the final contamination of stored zucchini purée. Based on a similarity threshold of 75%, the 134 B. cereus strains were distributed into 16 combined profile groups, and six strains remained unclustered (Fig. The BOX‐PCR in our study proved to be a sufficient and relatively rapid method for the detection of the two commonest Bacillus species found in milk and dairy products. R. All originated from soil and zucchinis. Rombouts Our study provides a method for distinguishing B. cereus and B. licheniformis strains sampled from the different sites of manufacturing process, the spore‐forming species which are commonly found in food samples. A. The remaining strains, representing groups G, I–L and unclustered strains BS, SM and SL′, had intermediate growth properties at low temperature and originated from soil and zucchinis. J. Isolated from Ready-to-Eat Foods and Powdered Milk in Day Care Centers in Colombia